DMPQ: . What is Polymerase Chain reaction? What are the materials required for Polymerase chain reaction?

:  The polymerase chain reaction is a technique for cloning a particular piece or amplifying a single or few copies of a piece of DNA, generating millions or more copies of that particular DNA sequence. One can make virtually unlimited copies of a single DNA molecule even though it may initially be present in a mixture containing many different DNA molecules.

 

MATERIAL REQUIRED

A basic PCR requires the following components:

  • DNAtemplatethat contains the DNA region called target to be amplified.
  • Two primerscomplementary to the DNA regions at the 5′ or 3′ end of DNA.
  • The enzyme, Taq polymeraseor another DNA polymerase with a temperature optimum at around 70°C.
  • Deoxynucleoside triphosphates (dNTPs)that are building blocks for DNA synthesis, e.g. dATP, dGTP, dCTP and dTTP.
  • Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
  • Divalent cations, magnesium or manganese ions; generally Mg2+ is used but Mn2+ can be utilized for PCR-mediated DNA mutagenesis.
  • Monovalent cationpotassium ions.

 

KPSC Notes brings Prelims and Mains programs for KPSC Prelims and KPSC Mains Exam preparation. Various Programs initiated by KPSC Notes are as follows:- For any doubt, Just leave us a Chat or Fill us a querry––

Hope we have satisfied your need for KPSC Prelims and Mains Preparation

Kindly review us to serve even better


KPSC Mains Test Series 2020

20 Quality mock tests and GS Mains Notes

Mains Test Series and Notes

Mains Printed Notes (With COD)


KPSC Prelims Test Series 2020

24 Quality mock tests and GS Prelims Notes

Prelims Test Series and Notes

Prelims Printed Notes (With COD)

Subscribe to KPSC Notes

Never Miss any KPSC important update!

Join 2,048 other subscribers

error: Content is protected !!