Biotechnology in agriculture
For about 10,000 years , farmers have been improving wild plants and animals through the selection and breeding of desirable characteristics. This breeding has resulted in the domesticated plants and animals that are commonly used in crop and livestock agriculture. In the twentieth century, breeding became more sophisticated, as the traits that breeders select for include increased yield, disease and pest resistance, drought resistance and enhanced flavor. Traits are passed from one generation to the next through genes, which are made of DNA. All living things—including the fruits, vegetables and meat that we eat—contain genes that tell cells how to function. Recently, scientists have learned enough to begin to identify and work with the genes (DNA) that are responsible for traits.
Agricultural biotechnology is a collection of scientific techniques used to improve plants, animals and microorganisms. Based on an understanding of DNA, scientists have developed solutions to increase agricultural productivity. Starting from the ability to identify genes that may confer advantages on certain crops, and the ability to work with such characteristics very precisely, biotechnology enhances breeders’ ability to make improvements in crops and livestock. Biotechnology enables improvements that are not possible with traditional crossing of related species alone.
Technological aspects of agricultural biotechnology
Genetic engineering
Scientists have learned how to move genes from one organism to another. This has been called genetic modification (GM), genetic engineering (GE) or genetic improvement (GI). Regardless of the name, the process allows the transfer of useful characteristics (such as resistance to a disease) into a plant, animal or microorganism by inserting genes (DNA) from another organism. Virtually all crops improved with transferred DNA (often called GM crops or GMOs) to date have been developed to aid farmers to increase productivity by reducing crop damage from weeds, diseases or insects.
Molecular markers
Traditional breeding involves selection of individual plants or animals based on visible or measurable traits. By examining the DNA of an organism, scientists can use molecular markers to select plants or animals that possess a desirable gene, even in the absence of a visible trait. Thus, breeding is more precise and efficient. For example, the International Institute of Tropical Agriculture has used molecular markers to obtain cowpea resistant to bruchid (a beetle), disease-resistant white yam and cassava resistant to Cassava Mosaic Disease, among others. Another use of molecular markers is to identify undesirable genes that can be eliminated in future generations.
Molecular diagnostics
Molecular diagnostics are methods to detect genes or gene products that are very precise and specific. Molecular diagnostics are used in agriculture to more accurately diagnose crop/livestock diseases.
Vaccines
Biotechnology-derived vaccines are used in livestock and humans. They may be cheaper, better and/or safer than traditional vaccines. They are also stable at room temperature, and do not need refrigerated storage; this is an important advantage for smallholders in tropical countries. Some are new vaccines, which offer protection for the first time against some infectious illnesses. For example, in the Philippines, biotechnology has been used to develop an improved vaccine to protect cattle and water buffalo against hemorrhagic septicemia, a leading cause of death for both species.
Tissue culture
Tissue culture is the regeneration of plants in the laboratory from disease-free plant parts. This technique allows for the reproduction of disease-free planting material for crops. Examples of crops produced using tissue culture include citrus, pineapples, avocados, mangoes, bananas, coffee and papaya.
Biofertilizers
Biofertilizers are defined as preparations containing living cells or latent cells of efficient strains of microorganisms that help crop plants’ uptake of nutrients by their interactions in the rhizosphere when applied through seed or soil. 
They accelerate certain microbial processes in the soil which augment the extent of availability of nutrients in a form easily assimilated by plants.
Very often microorganisms are not as efficient in natural surroundings as one would expect them to be and therefore artificially multiplied cultures of efficient selected microorganisms play a vital role in accelerating the microbial processes in soil.
Use of biofertilizers is one of the important components of integrated nutrient management, as they are cost effective and renewable source of plant nutrients to supplement the chemical fertilizers for sustainable agriculture. Several microorganisms and their association with crop plants are being exploited in the production of biofertilizers. They can be grouped in different ways based on their nature and function.
Different types of biofertilizers
Rhizobium
Rhizobium is a soil habitat bacterium, which can able to colonize the legume roots and fixes the atmospheric nitrogen symbiotically. The morphology and physiology of Rhizobium will vary from free-living condition to the bacteroid of nodules. They are the most efficient biofertilizer as per the quantity of nitrogen fixed concerned. They have seven genera and highly specific to form nodule in legumes, referred as cross inoculation group.
Rhizobium inoculant was first made in USA and commercialized by private enterprise in 1930s and the strange situation at that time has been chronicled by Fred.
Initially, due to absence of efficient bradyrhizobial strains in soil, soybean inoculation at that time resulted in bumper crops but incessant inoculation during the last four decades by US farmers has resulted in the build up of a plethora of inefficient strains in soil whose replacement by efficient strains of bradyrhizobia has become an insurmountable problem.
Azotobacter
Of the several species of Azotobacter, A. chroococcum happens to be the dominant inhabitant in arable soils capable of fixing N2 (2-15 mg N2 fixed /g of carbon source) in culture media.
The bacterium produces abundant slime which helps in soil aggregation. The numbers of A. chroococcum in Indian soils rarely exceeds 105/g soil due to lack of organic matter and the presence of antagonistic microorganisms in soil.
Azospirillum
Azospirillum lipoferum and A. brasilense (Spirillum lipoferum in earlier literature) are primary inhabitants of soil, the rhizosphere and intercellular spaces of root cortex of graminaceous plants.
They perform the associative symbiotic relation with the graminaceous plants. The bacteria of Genus Azospirillum are N2 fixing organisms isolated from the root and above ground parts of a variety of crop plants. They are Gram negative, Vibrio or Spirillum having abundant accumulation of polybetahydroxybutyrate (70 %) in cytoplasm.
Five species of Azospirillum have been described to date A. brasilense, A.lipoferum, A.amazonense, A.halopraeferens and A.irakense. The organism proliferates under both anaerobic and aerobic conditions but it is preferentially micro-aerophilic in the presence or absence of combined nitrogen in the medium.
Cyanobacteria
Both free-living as well as symbiotic cyanobacteria (blue green algae) have been harnessed in rice cultivation in India. A composite culture of BGA having heterocystous Nostoc, Anabaena, Aulosira etc. is given as primary inoculum in trays, polythene lined pots and later mass multiplied in the field for application as soil based flakes to the rice growing field at the rate of 10 kg/ha. The final product is not free from extraneous contaminants and not very often monitored for checking the presence of desiredalgal flora.
Once so much publicized as a biofertilizer for the rice crop, it has not presently attracted the attention of rice growers all over India except pockets in the Southern States, notably Tamil Nadu. The benefits due to algalization could be to the extent of 20-30 kg N/ha under ideal conditions but the labour oriented methodology for the preparation of BGA biofertilizer is in itself a limitation. Quality control measures are not usually followed except perhaps for random checking for the presence of desired species qualitatively.
Azolla Azolla is a free-floating water fern that floats in water and fixes atmospheric nitrogen in association with nitrogen fixing blue green alga Anabaena azollae. Azolla fronds consist of sporophyte with a floating rhizome and small overlapping bi-lobed leaves and roots. Rice growing areas in South East Asia and other third World countries have recently been evincing increased interest in the use of the symbiotic N2 fixing water fern Azolla either as an alternate nitrogen sources or as a supplement to commercial nitrogen fertilizers. Azolla is used as biofertilizer for wetland rice and it is known to contribute 40-60 kg N/ha per rice crop.
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